Shared Resources


Flow Cytometry

The mission of the Flow Cytometry Shared Resource is to enhance basic, preclinical and clinical cancer research. It is the goal of the shared resource to assist individual faculty members in their research endeavors, promoting interdisciplinary and inter-institutional research activities, and the education of graduate students, fellows and clinicians in the Cancer Center and its affiliated institutions. The shared resource is committed to providing professional, efficient, and reasonably priced Flow Cytometry Services.

  • Multicolor analyses and sorting, phenotyping, cloning of subpopulations of lymphocytes, tumor cells and (cancer) stem cells
  • Detection of apoptosis-related changes
  • Measurements of nitric oxide, hydrogen peroxide, free radicals, pH; Ca++ fluxes and fluxes of different dyes
  • Quantitation of multiple cytokines using fluorescent microspheres for multiplex detection of cytokines and chemokines
  • Assistance in writing flow cytometry methodologies for publications and grant applications
  • Training sessions for self operation

To learn more and utilize this resource click here.


Contacts


Daniel, Benjamin Director
Office: 5.044V
Phone: (210) 567-3911
Email: danielb@uthscsa.edu


Rebel, Vivienne Co-Director
Office: 3.100.20
Phone: (210) 562-9096
Email: rebel@uthscsa.edu


Gorena, Karla Manager
Office: 5.044V
Phone: (210) 567-3911
Email: moncadak@uthscsa.edu


Genomics

A genomics approach to cancer research is key to the discovery and validation of new biomarkers that can be applied to the diagnosis, prognosis and treatment response evaluation in cancer. The Genomics Shared Resource provides services on a number of platforms to aid the researcher in genomic analyses. The major service areas relevant to CTRC members are:

  • Genotyping and copy number variant analysis:
    • Custom Illumina GoldenGate 48-plex to 384-plex single nucleotide polymorphism (SNP) genotyping for any species
    • Illumina Infinium whole genome SNP arrays for several species
    • TaqMan allelic discrimination assays and copy number determination assays
    • Illumina mouse medium density linkage panel analysis for speed congenics
  • Gene expression analysis
    • Illumina whole genome gene expression beadchip profiling for human and mouse
    • Quantitative real-time PCR
  • Amplicon Sequencing using Life Technologies Ion Torrent PGM
    • Mutational analysis of select cancer genes using Ion Ampliseq cancer panels
    • Viral and microbiome characterization
  • Biobanking and sample preparation services
    • Isolation of buffy coats and cryopreservation
    • Epstein-Barr virus immortalization of human lymphocytes
    • DNA and RNA isolation from biologic samples
    • Human cell line authentication

To learn more and utilize this resource click here.


Contacts

Leach, Robin Director
Office: 552C
Phone: (210) 567-6947
Fax: (210) 567-6781
Email: leach@uthscsa.edu


Johnson-Pais, Teresa Co-Director
Office: 528L
Phone: (210) 567-6571
Fax: (210) 567-6781
Email: paist@uthscsa.edu


Livi, Carolina Gene expression Co-Director
Office: 300.78 STRF or 3.040 HHB/IBT/TRP
Phone: (210) 567-7237
Fax: (210) 567-7277
Email: livi@uthscsa.edu


Grayson, Marcia Staff
Office: 573C MED
Phone: (210) 567-0950
Email: grayson@uthscsa.edu



Macromolecular Interactions

The Macromolecular Interactions Shared Resource provides state-of-the-art resources to members of the Cancer Center enabling the solution state analysis of biological macromolecules that play critical roles in cancer from the basic science level through drug discovery and development.

  • Solution-based characterization of dynamic protein, nucleic acid,lipid and small molecule interactions
  • Quantitative interaction measurements: kinetics, thermo/hydrodynamics, stoichiometry, and assembly state
  • Four complementary technologies in a single resource: Surface Plasmon resonance (Biacore T100/3000), analytical ultracentrifugation (interference, absorbance and fluorescence detection), isothermal titration calorimetry and dynamic and static multi-angle light scattering
  • We offer comprehensive service including experimental design, data collection and analysis, grant and manuscript preparation assistance, and user training

To learn more and utilize this resource click here.


Contacts


Lafer, Eileen Co-Director
Office: 415B
Phone: (210) 567-3764
Email: lafer@biochem.uthscsa.edu


Demeler, Borries Co-Director
Office: 420-D
Phone: (210) 767-3332
Fax: (210) 567-1136
Email: demeler@biochem.uthscsa.edu


Schirf, Virgil Technical Director
Office: 414B/421D
Phone: (210) 767-3371
Fax: 567-6595
Email: schirfv@biochem.uthscsa.edu


Macromolecular Structure

The Macromolecular Structure Shared Resource (MSSR) is comprised of the X-ray crystallography (X-ray) and the Nuclear Magnetic Resonance (NMR) laboratories.


NMR


To learn more and utilize this resource click here.


Contacts


Hinck, Andrew Director
Office: AHB 5.206
Phone: (210) 567-8780
Fax: (210) 567-8778
Email: hinck@uthscsa.edu


Browning, Martha Staff
Office: 425B
Phone: (210) 567-0431
Fax: (210) 567-6595
Email: browningm@uthscsa.edu


Hinck, Cynthia Staff
Office: 5.304 AHR
Phone: (210) 567-8030
Fax: (210) 567-8778
Email: hinckc@biochem.uthscsa.edu


X-Ray


To learn more and utilize this resource click here.


Contacts


Hart, John Director
Office: 542C.2
Phone: (210) 567-0751
Fax: (210) 567-6595
Email: pjhart@biochem.uthscsa.edu


Taylor, Alex Technical Director
Office: 545C
Phone: (210) 567-3781
Fax: (210) 567-6595
Email: taylor@biochem.uthscsa.edu


Holloway, Stephen Staff
Office: 545C
Phone: (210) 567-3767
Fax: (210) 567-6595
Email: mirabilis@biochem.uthscsa.edu


To learn more and utilize this resource click here.


Mass Spectrometry

The Mass Spectrometry Shared Resource offers a wide range of analytical capabilities to support the needs of Cancer Center investigators and other researchers at UTHSCSA and in the nearby community.


Mass spectrometry is essential for a wide variety of investigations that are part of the Cancer Center programs.


To learn more and utilize this resource click here.


Contacts


Weintraub, Susan Director
Office: 435D
Phone: (210) 567-4043
Fax: (210) 567-5524
Email: weintraub@uthscsa.edu


Gao, Xiaoli Technical Director
Office: STRF 256
Phone: (210) 562-4067
Email: gaox@uthscsa.edu


Hakala, Kevin Staff
Office: 428B
Phone: (210) 567-4159
Fax: (210) 567-5524
Email: hakala@uthscsa.edu


Pardo, Sammy Staff
Office: 428B
Phone: (210) 567-6736
Email: pardo@uthscsa.edu


Optical Imaging

The Optical Imaging Shared Resource provides ongoing service to the Cancer Center and its members by providing access to state-of-the-art optical imaging equipment, including multiparameter digital imaging workstations, confocal microscopes, and multiphoton microscopes. Highly experienced personnel in the shared resource are available to consult with users of the facility regarding the appropriate instrumentation or imaging technique for their specific needs.


  • Provide access to state-of-the-art optical microscopy instrumentation and image acquisition systems.
  • Maintain and test optical microscopy instrumentation to ensure it is in proper working order and available for investigators.
  • Provide consultation and training regarding the theory, application, and operation of optical imaging instrumentation.
  • Provide technical assistance and perform imaging upon request.
  • Assist in preparation of grant applications and publications.
  • Provide letters of support for grant applications to confirm the availability of needed technology.

To learn more and utilize this resource click here.


Contacts


Lechleiter, James Director
Office: 516D
Phone: (210) 567-6254
Fax: (210) 567-6781
Email: lechleiter@uthscsa.edu


Wang, Exing Technical Director
Office: STRF 252
Phone: (210) 562-4062
Email: wange3@uthscsa.edu


Wewer, Jimmy Staff
Office: 2.514U
Phone: (210) 567-3152
Email: wewer@uthscsa.edu


Pathology

The mission of the Pathology Shared Resource is to provide technical and professional expertise in the handling of human and animal tissues. By interacting with the shared resource facility, P30 members are educated in how histologic approaches in experimental design can enhance their research. The P30 tissue/tumor bank makes appropriate tissue/tumor bank specimens available to P30 members to generate and test hypotheses in vivo in humans.

  • Histology/histochemistry (paraffin block preparation and H&E slides)
  • Special histochemistry
  • Immunohistochemistry
  • Apoptosis assays (TUNEL and caspase)
  • Tumor banking (frozen and paraffin banks)
  • Laser microdissection
  • Tumor array construction and usage
  • Consultation/collaboration with experienced pathologists

Contacts


Pathology Shared Resource Director
Robert Reddick, MD
Phone: (210) 567-4001
Email: reddick@uthscsa.edu


Laboratory

Phone:  (210) 567-5066

Email:CTRCPathology@uthscsa.edu or tissuebank@uthscsa.edu

Biostatistics and Informatics

The Biostatistics and Informatics Shared Resource (BISR) provides biostatistics and informatics resources to the Cancer Therapy and Research Center (P30) at the University of Texas Health Science Center at San Antonio (UTHSCSA). This centralized shared resource provides flexible, cost-efficient services to basic, translational, clinical and population-based researchers by providing biostatistical consultation in study design, statistical analysis and interpretation of research findings as well as support for data collection and information management. The BISR maintains the cancer center informatics infrastructure and assists individual investigators or groups with the database design, data security, and research computing needs. The BISR supports the data management personnel of the Office of Research Administration (ORA). Informatics solutions have streamlined many regulatory operations such as quality assurance review, automated reporting of adverse events, and accrual monitoring. BISR biostatisticians are active members of the Protocol Review Committee and the Data Safety Monitoring Committee.


Biostatistics

  • Biostatistical review of all CTRC human subjects research protocols
  • Accrual, safety and efficacy monitoring
  • Study design and statistical analysis consultation
  • Collaborate on the development of grants

Informatics

  • Database applications for clinical and biological information management
  • Implement standardized quality control procedures
  • Facilitate compliance with regulatory agency reporting requirements
  • Develop database linkages with specimen, clinical, and population-based repositories
  • Provide data services to support CTRC strategic planning
  • Provide bioinformatics consultation

To learn more and utilize this resource click here


Contacts:


Director:
Brad Pollock, MPH, PhD
E-mail: bpollock@uthscsa.edu
Phone: (210) 567-0836


Biostatistics Co-Director:
Joel Michalek, PhD
E-mail: Michalek@uthscsa.edu
Phone: (210) 567-0836


Informatics Co-Director:
Bill Sanns, BSG
E-mail: sanns@uthscsa.edu
Phone: (210) 450-1470


Bioinformatics Co-Director:
Yidong Chen, PhD
E-mail: ChenY8@uthscsa.edu
Phone: (210) 562-9168



Next Generation Sequencing

By deploying next generation sequencing system, the mission of the Next Generation Sequencing Shared Resource is to effectively bring Cancer Center's genomic research capability into the cutting-edge genomic era, greatly enhancing the research competitiveness, promoting cancer genomics education, and most importantly, significantly enhancing our impact in cancer patient treatment and care.


The Next Generation Sequencing Shared Resource performs all protocols necessary for preparing a biological sample to be sequenced on the Illumina HiSeq 2000 system. We have experience in customizing experiments and developing new sequencing applications. The shared resource is committed to providing professional, efficient, and reasonably priced next generation sequencing services with following major application:

  • DNA analysis (whole genome de novo sequencing, re-sequencing, exon and target sequencing), transcriptome analysis (mRNA-Seq and Small RNA-Seq)
  • Methylation analysis (MBDCap-Seq)
  • Epigenetics analysis (ChIP-Seq and RIP-Seq)
  • Basic bioinformatics services
  • Assistance in writing NGS methodologies for publications and grant applications

To learn more and utilizing this resource, click here.


Contacts

Lai, Zhao Director
Office: GCCRI 4.100.14
Phone: (210) 562-9246
Fax: (210) 562-9135
Email: laiz@uthscsa.edu


Garcia, Dawn Technical Director
Office: GCCRI 2.120
Phone: (210) 562-9222
Email: garcia1@uthscsa.edu


Chen, Yidong Faculty Advisor, Bioinformatics analysis
Office: GCCRI/4.100.06
Phone: (210) 562-9163
Fax: (210) 562-9135
Email: cheny8@uthscsa.edu