Shared Resources


Flow Cytometry

The mission of the Flow Cytometry Shared Resource is to enhance basic, preclinical and clinical cancer research. It is the goal of this shared resource to assist individual faculty members in their research endeavors, promoting interdisciplinary and inter-institutional research activities, and the education of graduate students, fellows and clinicians in the Cancer Center and its affiliated institutions. We are committed to providing professional, efficient, and reasonably priced flow cytometry services.


Services include:

  • Multi-parameter sorting and analysis of lymphocytes, tumor cells, [cancer] stem cells, or any other cell type that can be fluorescently labeled.
  • Single cell sorting for sub-cloning and single cell genomic assays
  • Multi-parameter analysis of cellular functions such as cell cycle, apoptosis, and membrane potential.
  • Quantitation of cytokines and chemokines using multiplex fluorescent microspheres.
  • Assistance in writing flow cytometry methodologies for publications and grant applications
  • Training sessions for self operation

To learn more and utilize this resource click here.


Contacts


Benjamin Daniel, Director
Office: 5.044V
Phone: (210) 567-3911
Email: danielb@uthscsa.edu


Vivienne Rebel, Scientific Advisor
Office: 3.100.20
Phone: (210) 562-9096
Email: rebel@uthscsa.edu


Karla Gorena, Technical Director
Office: 5.044V
Phone: (210) 567-3911
Email: moncadak@uthscsa.edu


Genomics

A genomics approach to cancer research is key to the discovery and validation of new biomarkers that can be applied to the diagnosis, prognosis and treatment response evaluation in cancer. The Genomics Shared Resource provides services on a number of platforms to aid the researcher in genomic analyses. The major service areas relevant to CTRC members are:

  • Genotyping and copy number variant analysis:
    • Custom Illumina GoldenGate 48-plex to 384-plex single nucleotide polymorphism (SNP) genotyping for any species
    • Illumina Infinium whole genome SNP arrays for several species
    • TaqMan allelic discrimination assays and copy number determination assays
    • Illumina mouse medium density linkage panel analysis for speed congenics
  • Gene expression analysis
    • Illumina whole genome gene expression beadchip profiling for human and mouse
    • Quantitative real-time PCR
  • Amplicon Sequencing using Life Technologies Ion Torrent PGM
    • Mutational analysis of select cancer genes using Ion Ampliseq cancer panels
    • Viral and microbiome characterization
  • Biobanking and sample preparation services
    • Isolation of buffy coats and cryopreservation
    • Epstein-Barr virus immortalization of human lymphocytes
    • DNA and RNA isolation from biologic samples
    • Human cell line authentication

To learn more and utilize this resource click here.


Contacts

Robin Leach, Director
Office: 552C
Phone: (210) 567-6947
Fax: (210) 567-6781
Email: leach@uthscsa.edu


Teresa Johnson-Pais, Co-Director
Office: 528L
Phone: (210) 567-6571
Fax: (210) 567-6781
Email: paist@uthscsa.edu


Marcia Grayson, Staff
Office: 573C MED
Phone: (210) 567-0950
Email: grayson@uthscsa.edu



Macromolecular Structure and Interactions

The mission of the Macromolecular Structure and Interactions Shared Resource (MSISR) is to provide CTRC members with state-of-the-art resources for investigating the molecular mechanisms by which cancer-related macromolecule function and for developing novel targeted therapies for cancer treatment. The MSISR is comprised of the X-ray crystallography (X-ray), Nuclear Magnetic Resonance (NMR), and Macromolecular Interactions (MMI) component laboratories, which provide a complementary set of tools for determining macromolecular structures and investigating macromolecular interactions. 


The three component laboratories of the MSISR offer consultation, guidance, and assistance with experimental design, sample preparation, data acquisition, interpretation, and presentation. The component laboratories also offer workshops and provide web-based services, including tutorials and software guides, to educate users and about technical aspects and capabilities. The specialized services and expertise offered by the component laboratories are as follows


The overall scientific oversight for the MSISR is provided by Dr. Andrew Hinck, a structural biologist whose research is heavily focused in the cancer arena, while oversight of the component laboratories are provided by tenured faculty with expertise in these areas (Andrew Hinck – NMR, P. John Hart – X-ray, and Eileen Lafer – MMI).    


Andrew Hinck, MSISR  Director
Office: RAB 5.206
Phone: (210) 567-8780
Fax: (210) 567-8778
Email: hinck@uthscsa.edu


Eileen Lafer, MSISR Co-Director
Office: 415B
Phone: (210) 567-3764
Email: lafer@biochem.uthscsa.edu


John Hart, MSISR Co-Director
Office: 542C.2
Phone: (210) 567-0751
Fax: (210) 567-6595
Email: pjhart@biochem.uthscsa.edu

Macromolecular Interactions Laboratory

The Macromolecular Interactions Laboratory provides state-of-the-art resources to members of the Cancer Center enabling the solution state analysis of biological macromolecules that play critical roles in cancer from the basic science level through drug discovery and development.

  • Solution-based characterization of dynamic protein, nucleic acid,lipid and small molecule interactions
  • Quantitative interaction measurements: kinetics, thermo/hydrodynamics, stoichiometry, and assembly state
  • Four complementary technologies in a single resource: Surface Plasmon resonance (Biacore T100/3000), analytical ultracentrifugation (interference, absorbance and fluorescence detection), isothermal titration calorimetry and dynamic and static multi-angle light scattering
  • We offer comprehensive service including experimental design, data collection and analysis, grant and manuscript preparation assistance, and user training

Eileen Lafer, MSISR Co-Director
Office: 415B
Phone: (210) 567-3764
Email: lafer@biochem.uthscsa.edu


Virgil Schirf, MSISR Technical Director
Office: 414B/421D
Phone: (210) 767-3371
Fax: 567-6595
Email: schirfv@biochem.uthscsa.edu

To learn more and utilize this resource click here (http://cmmi.uthscsa.edu/)

 

NMR and X-ray  Laboratories

The X-ray laboratory provides capabilities for determining atomic resolution structures of cancer-related macromolecules, regardless of size. The NMR laboratory also provides capabilities for determining the structures of macromolecules, but unlike X-ray methods, it is only possible to determine structures that are smaller than about 50 kDa. The atomic resolution structures determined by X-ray and NMR methods provide detailed insights as to how cancer-related macromolecules function, and in many cases, how they might be inhibited to block their tumor- and metastasis-promoting activities. The X-ray and NMR laboratories also provide capabilities for discovery of new targeted therapies; for example, they can be used to determine the structure of hit molecules discovered by high throughput screening (HTS) bound to their macromolecular targets in an effort to discover how they are binding and how binding might be improved. The X-ray and NMR laboratories also have the capability to discover novel targeted therapies using fragment based drug discovery (FBDD), a new approach for drug discovery in which high potency inhibitors are generated by coupling together fragments that bind adjacent to one another.


The capabilities and contacts for the NMR laboratory are as follows:

  • Automated preparation of NMR samples, including addition of compounds from the Maybridge Ro3 fragment library, using the Gilson 213 liquid handler.
  • Acquisition of NMR data, including automated acquisition, with hundreds of NMR samples
  • Assistance with NMR data processing, analysis, and three dimensional structure determination.
  • Assistance with titrations of isotope labeled macromolecules with unlabeled binding partners to map interaction surfaces with other macromolecules, ligands, and small molecule therapeutics. 


Andrew Hinck, Director
Office: RAB 5.206
Phone: (210) 567-8780
Fax: (210) 567-8778
Email: hinck@uthscsa.edu


Udayar Ilangovan, Technical Director
Office: RAB 5.210
Phone: (210) 567-8706
Fax: (210) 567-8778
Email: ilangovan@uthscsa.edu


To learn more and utilize the NMR resource click here (http://nmr.uthscsa.edu)


The capabilities and contacts for the X-ray laboratory are as follows:

  • Automated setup and imaging of vapor diffusion or microbatch crystallizations trials.
  • Automated addition of addition of compounds from the Maybridge Ro3 fragment library to already formed crystals.
  • Acquisition of X-ray diffraction data, including fragment screens, with hundreds of crystals.
  • Assistance with X-ray data processing, macromolecular model building, and structure refinement.

John Hart, Director
Office: 542C.2
Phone: (210) 567-0751
Fax: (210) 567-6595
Email: pjhart@biochem.uthscsa.edu


Alex Taylor, Technical Director
Office: 545C
Phone: (210) 567-3781
Fax: (210) 567-6595
Email: taylor@biochem.uthscsa.edu
To learn more and utilize the X-ray resource click here (http://xray.uthscsa.edu)


Mass Spectrometry

The Mass Spectrometry Shared Resource offers a wide range of analytical capabilities to support the needs of Cancer Center investigators and other researchers at UTHSCSA and in the nearby community.


Mass spectrometry is essential for a wide variety of investigations that are part of the Cancer Center programs.


To learn more and utilize this resource click here.


Contacts


Susan Weintraub, Director
Office: 435D
Phone: (210) 567-4043
Fax: (210) 567-5524
Email: weintraub@uthscsa.edu


Xiaoli Gao, Technical Director
Office: STRF 256
Phone: (210) 562-4067
Email: gaox@uthscsa.edu


Kevin Hakala, Staff
Office: 428B
Phone: (210) 567-4159
Fax: (210) 567-5524
Email: hakala@uthscsa.edu


Sammy Pardo, Staff
Office: 428B
Phone: (210) 567-6736
Email: pardo@uthscsa.edu


Optical Imaging

The Optical Imaging Shared Resource provides ongoing service to the Cancer Center and its members by providing access to state-of-the-art optical imaging equipment, including multiparameter digital imaging workstations, confocal microscopes, and multiphoton microscopes. Highly experienced personnel in the shared resource are available to consult with users of the facility regarding the appropriate instrumentation or imaging technique for their specific needs.


  • Provide access to state-of-the-art optical microscopy instrumentation and image acquisition systems.
  • Maintain and test optical microscopy instrumentation to ensure it is in proper working order and available for investigators.
  • Provide consultation and training regarding the theory, application, and operation of optical imaging instrumentation.
  • Provide technical assistance and perform imaging upon request.
  • Assist in preparation of grant applications and publications.
  • Provide letters of support for grant applications to confirm the availability of needed technology.

To learn more and utilize this resource click here.


Contacts


James Lechleiter, Director
Office: 516D
Phone: (210) 567-6254
Fax: (210) 567-6781
Email: lechleiter@uthscsa.edu


Exing Wang, Co-Director
Office: STRF 252
Phone: (210) 562-4062
Email: wange3@uthscsa.edu


Jimmy Wewer, Staff
Office: 2.514U
Phone: (210) 567-3152
Email: wewer@uthscsa.edu


Pathology

The mission of the Pathology Shared Resource is to provide technical and professional expertise in the handling of human and animal tissues. By interacting with the shared resource facility, P30 members are educated in how histologic approaches in experimental design can enhance their research. The P30 tissue/tumor bank makes appropriate tissue/tumor bank specimens available to P30 members to generate and test hypotheses in vivo in humans.

  • Histology/histochemistry (paraffin block preparation and H&E slides)
  • Special histochemistry
  • Immunohistochemistry
  • Apoptosis assays (TUNEL and caspase)
  • Tumor banking (frozen and paraffin banks)
  • Laser microdissection
  • Tumor array construction and usage
  • Consultation/collaboration with experienced pathologists

Contacts


Pathology Shared Resource Director
Robert Reddick, MD
Phone: (210) 567-4001
Email: reddick@uthscsa.edu


Laboratory

Phone:  (210) 567-5066

Email:CTRCPathology@uthscsa.edu or tissuebank@uthscsa.edu

Biostatistics and Informatics

The Biostatistics and Informatics Shared Resource (BISR) provides biostatistics and informatics resources to the Cancer Therapy and Research Center (P30) at the University of Texas Health Science Center at San Antonio (UTHSCSA). This centralized shared resource provides flexible, cost-efficient services to basic, translational, clinical and population-based researchers by providing biostatistical consultation in study design, statistical analysis and interpretation of research findings as well as support for data collection and information management. The BISR maintains the cancer center informatics infrastructure and assists individual investigators or groups with the database design, data security, and research computing needs. The BISR supports the data management personnel of the Office of Research Administration (ORA). Informatics solutions have streamlined many regulatory operations such as quality assurance review, automated reporting of adverse events, and accrual monitoring. BISR biostatisticians are active members of the Protocol Review Committee and the Data Safety Monitoring Committee.


Biostatistics

  • Biostatistical review of all CTRC human subjects research protocols
  • Accrual, safety and efficacy monitoring
  • Study design and statistical analysis consultation
  • Collaborate on the development of grants

Informatics

  • Database applications for clinical and biological information management
  • Implement standardized quality control procedures
  • Facilitate compliance with regulatory agency reporting requirements
  • Develop database linkages with specimen, clinical, and population-based repositories
  • Provide data services to support CTRC strategic planning
  • Provide bioinformatics consultation

To learn more and utilize this resource click here


Contacts:



Brad Pollock, MPH, PhD, Director
E-mail: bpollock@uthscsa.edu
Phone: (210) 567-0836


Joel Michalek, PhD, Biostatistics Co-Director
E-mail: Michalek@uthscsa.edu
Phone: (210) 567-0836


Bill Sanns, BSG, Informatics Co-Director
E-mail: sanns@uthscsa.edu
Phone: (210) 450-1470


Yidong Chen, PhD, Bioinformatics Co-Director
E-mail: ChenY8@uthscsa.edu
Phone: (210) 562-9168

Next Generation Sequencing

By deploying next generation sequencing system, the mission of the Next Generation Sequencing Shared Resource is to effectively bring Cancer Center's genomic research capability into the cutting-edge genomic era, greatly enhancing the research competitiveness, promoting cancer genomics education, and most importantly, significantly enhancing our impact in cancer patient treatment and care.


The Next Generation Sequencing Shared Resource performs all protocols necessary for preparing a biological sample to be sequenced on the Illumina HiSeq 2000 system. We have experience in customizing experiments and developing new sequencing applications. The shared resource is committed to providing professional, efficient, and reasonably priced next generation sequencing services with following major application:

  • DNA analysis (whole genome de novo sequencing, re-sequencing, exon and target sequencing), transcriptome analysis (mRNA-Seq and Small RNA-Seq)
  • Methylation analysis (MBDCap-Seq)
  • Epigenetics analysis (ChIP-Seq and RIP-Seq)
  • Basic bioinformatics services
  • Assistance in writing NGS methodologies for publications and grant applications

To learn more and utilizing this resource, click here.


Contacts

Zhao Lai, Director
Office: GCCRI 4.100.14
Phone: (210) 562-9246
Fax: (210) 562-9135
Email: laiz@uthscsa.edu


Dawn Garcia, Technical Director
Office: GCCRI 2.120
Phone: (210) 562-9222
Email: garcia1@uthscsa.edu


Yidong Chen, Faculty Advisor, Bioinformatics analysis
Office: GCCRI/4.100.06
Phone: (210) 562-9163
Fax: (210) 562-9135
Email: cheny8@uthscsa.edu